The Gadsden flag, featuring a rattlesnake and the word "Don't Tread on Me

https://remingtonfseq702.stonefielddigest.com/posts/the-legacy-of-civil-war-flags-symbolism-and-design-throughout-history-2

The Gadsden flag, presenting a rattlesnake and the phrase "Don't Tread on Me," was once adopted by a few Confederate devices as a symbol of resistance opposed to perceived oppression.